کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7608182 1493374 2018 32 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Immobilization of a phosphonium ionic liquid on a silica monolith for hydrophilic interaction chromatography
ترجمه فارسی عنوان
امولسیون یک مایع یون فسفونیوم روی یک سیلیس مونولیت برای کروماتوگرافی تعامل هیدرولیکی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A methodology for preparing phosphonium-based ionic liquid modified silica-based monolithic capillary columns is presented. The silica monolithic columns with dimensions of 150 × 0.1 mm were modified by a phosphonium-based ionic liquid (trioctyl(3/4-vinylbenzyl)phosphonium chloride) via 3-(trimethoxysilyl)propyl methacrylate. The prepared columns were evaluated under hydrophilic interaction liquid chromatography separation conditions, employing a sample mixture containing purine and pyrimidine bases and nucleosides. Detection was made by UV. The high efficiency of the original silica monolith was preserved even after modification, and it reached values in the range of 98,000-174,000 theoretical plates/m. The effects of the concentration of acetonitrile in the mobile phase, the presence of additives in the mobile phase, such as, acetic acid or ammonium acetate, and the pH of the mobile phase on the separation of some selected analytes were investigated. The prepared columns showed different separation selectivity compared to silica, phenyl and sulfobetaine stationary phases.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1552, 1 June 2018, Pages 53-59
نویسندگان
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