کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7608535 | 1493383 | 2018 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cyclodextrin based polymer sorbents for micro-solid phase extraction followed by liquid chromatography tandem mass spectrometry in determination of endogenous steroids
ترجمه فارسی عنوان
سوربنت های پلیمری بر پایه سیکلوکودکستر برای استخراج فاز میکرو جامد و سپس با استفاده از اسپکترومتری جرمی کروماتوگرافی مایع در تعیین استروئید های اندوژن
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Sorbents were prepared by cross-linking β-cyclodextrin (β-CD) using two different types of cross-linker units at variable reactant mole ratios. The resulting polymers containing β-CD were evaluated as sorbents in micro-solid phase extraction (μ-SPE) format for the extraction of the endogenous steroids testosterone (T), epitestosterone (E), androsterone (A), etiocholanolone (Etio), 5α-androstane-3α,17β-diol (5αAdiol) and 5β-androstane-3α,17β-diol (5βAdiol). The best sorbent (C1; cyclodextrin polymer) showed superior extraction characteristics compared with commercial sorbents (C18 and Bond Elut Plexa). Parameters influencing the extraction efficiency of the C1 sorbent such as extraction and desorption times, desorption solvent and volume of sample were investigated. The extracts were separated using a Hypersil Gold column (50â¯Ãâ¯2.1â¯mm, 1.9â¯Î¼m) under gradient elution coupled to a LC-MS/MS. The compounds were successfully separated within 8â¯min. The method offers good repeatability (RSDâ¯<â¯10%) and linearity (r2â¯>â¯0.995) were within the range of 1-200â¯ngâ¯mLâ1 for T and E, 250-4000â¯ngâ¯mLâ1 for A and Etio and 25-500â¯ngâ¯mLâ1 for 5αAdiol and 5βAdiol, respectively. The method was applied for the determination of steroid profile of urine from volunteers.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1543, 30 March 2018, Pages 23-33
Journal: Journal of Chromatography A - Volume 1543, 30 March 2018, Pages 23-33
نویسندگان
Normaliza Abdul Manaf, Bahruddin Saad, Mohamed H. Mohamed, Lee D. Wilson, Aishah A. Latiff,