کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7608682 1493386 2018 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Thermally condensed humic acids onto silica as SPE for effective enrichment of glucocorticoids from environmental waters followed by HPLC-HESI-MS/MS
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Thermally condensed humic acids onto silica as SPE for effective enrichment of glucocorticoids from environmental waters followed by HPLC-HESI-MS/MS
چکیده انگلیسی
Pristine humic acids (HAs) were thermally condensed onto silica microparticles by a one-pot, inexpensive and green preparation route obtaining a mixed-mode sorbent (HA-C@silica) with good sorption affinity for glucocorticoids (GCs). The carbon-based material, characterized by various techniques, was indeed applied as the sorbent for fixed-bed solid-phase extraction of eight GCs from river water and wastewater treatment plant effluent, spiked at different concentration levels in the range 1-400 ng L−1. After sample extraction, the target analytes were simultaneously and quantitatively eluted in a single fraction of methanol, achieving enrichment factor 4000 and 1000 in river water and wastewater effluent, respectively. Full recovery for all compounds, was gained in the real matrices studied (80-125% in river water, 79-126% in wastewater effluent), with inter-day precision showing relative standard deviations (RSD) below 15% and 18% (n = 3), for river and wastewater effluent, correspondingly. The high enrichment factors coupled with high-performance liquid chromatography tandem mass spectrometry quantification (MRM mode) provided method quantification limits of 0.009-0.48 ng L−1 in river water and 0.06-3 ng L−1 in wastewater effluent and, at the same time, secure identification of the selected drugs. As also evidenced by comparison with literature, HA-C@silica proved to be a valid alternative to the current commercial sorbents, in terms of extraction capability, enrichment factor, ease of preparation and cost. The batch-to-batch reproducibility was assessed by recovery tests on three independently prepared HA-C@silica powders (RSD lower than 7%).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1540, 9 March 2018, Pages 38-46
نویسندگان
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