کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7608798 | 1493388 | 2018 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Separation and quantitation of eight isomers in a molecule with three stereogenic centers by normal phase liquid chromatography
ترجمه فارسی عنوان
جداسازی و کم کردن هشت ایزومر در یک مولکول با سه مرکز استروژنیک توسط کروماتوگرافی مایع فاز فازی
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
The separation and detection of these stereoisomers was achieved using a Chiralcel OD-H, 4.6â¯Ãâ¯250â¯mm, 5â¯Î¼m dp column with heptane: ethanol: dichloromethane in a ratio of 95:3:2 (v:v:v) as mobile phase at a flow rate of 0.7â¯mL/min. UV detection was carried out at 245â¯nm and the column temperature was maintained at 15â¯Â°C. The analytical method was phase appropriately validated. The limit of detection and limit of quantification were found to be 0.035 and 0.07â¯Î¼g, respectively. The newly developed method has been implemented for routine utilization to monitor the chiral control during process development and used as the quality control method for chiral purity of the desired compound.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1538, 23 February 2018, Pages 108-111
Journal: Journal of Chromatography A - Volume 1538, 23 February 2018, Pages 108-111
نویسندگان
Nilesh Joshi, Balaji Dhamarlapati, Athimoolam Pillai, Justine Paulose, Jessica Tan, Laura E. Blue, Jason Tedrow, Bob Farrell,