کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7608819 1493389 2018 29 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Scalability of pre-packed preparative chromatography columns with different diameters and lengths taking into account extra column effects
ترجمه فارسی عنوان
مقیاس پذیری ستون های کروماتوگرافی تهیه شده از پیش آماده شده با قطر و طول متفاوت با توجه به اثرات ستون اضافی
کلمات کلیدی
گسترش باند، ابعاد ستون، عملکرد ستون، صلاحیت ستون، دینامیک سیالات محاسباتی، ایستگاه کاری کروماتوگرافی،
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Small pre-packed columns are commonly used to estimate the optimum run parameters for pilot and production scale. The question arises if the experiments obtained with these columns are scalable, because there are substantial changes in extra column volume when going from a very small scale to a benchtop column. In this study we demonstrate the scalability of pre-packed disposable and non-disposable columns of volumes in the range of 0.2-20 ml packed with various media using superficial velocities in the range of 30-500 cm/h. We found that the relative contribution of extra column band broadening to total band broadening was not only high for columns with small diameters, but also for columns with a larger volume due to their wider diameter. The extra column band broadening can be more than 50% for columns with volumes larger than 10 ml. An increase in column diameter leads to high additional extra column band broadening in the filter, frits, and adapters of the columns. We found a linear relationship between intra column band broadening and column length, which increased stepwise with increases in column diameter. This effect was also corroborated by CFD simulation. The intra column band broadening was the same for columns packed with different media. An empirical engineering equation and the data gained from the extra column effects allowed us to predict the intra, extra, and total column band broadening just from column length, diameter, and flow rate.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1537, 16 February 2018, Pages 66-74
نویسندگان
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