کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7609148 | 1493393 | 2018 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Thin-layer chromatography combined with diode laser thermal vaporization inductively coupled plasma mass spectrometry for the determination of selenomethionine and selenocysteine in algae and yeast
ترجمه فارسی عنوان
کروماتوگرافی لایه نازک همراه با تبخیر حرارتی لیزر دیود، طیف سنجی جرمی پلاسما همراه با تشخیص سلنیوم متینین و سلنوسیستین در جلبکها و مخمر
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
In this work we present a simple and cost-effective approach for the determination of selenium species in algae and yeast biomass, based on a combination of thin-layer chromatography (TLC) with diode laser thermal vaporization inductively coupled plasma mass spectrometry (DLTV ICP MS). Extraction of freeze-dried biomass was performed in 4M methanesulphonic acid and the selenium species were vaporized from cellulose TLC plates employing a continuous-wave infrared diode laser with power up to 4â¯W using a simple laboratory-built apparatus. Selenomethionine and selenocysteine were quantified with limits of detection 3â¯Î¼gâ¯Lâ1 in a Se-enriched microalgae Chlorella vulgaris and yeast certified reference material SELM-1. Results delivered by TLC-DLTV ICP MS were consistent with those obtained by a routine coupling of high-performance liquid chromatography (HPLC) to ICP MS. In addition, the TLC approach is capable of analyzing extract containing even undiluted crude hydrolysates that could damage HPLC columns.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1533, 19 January 2018, Pages 199-207
Journal: Journal of Chromatography A - Volume 1533, 19 January 2018, Pages 199-207
نویسندگان
AntonÃn BednaÅÃk, Jan Kuta, Dai Long Vu, KarolÃna Ranglová, Pavel Hrouzek, Viktor Kanický, Jan Preisler,