کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7609351 1493396 2017 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Mechanical characterisation of agarose-based chromatography resins for biopharmaceutical manufacture
ترجمه فارسی عنوان
مشخصات مکانیکی رزین های کروماتوگرافی مبتنی بر آگارز برای تولید بیوفرمارکت
کلمات کلیدی
مشخصات مکانیکی، رسانه های کروماتوگرافی، دانه های آگاروز، فشار جریان، تجزیه و تحلیل مکانیکی پویا،
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Mechanical characterisation of agarose-based resins is an important factor in ensuring robust chromatographic performance in the manufacture of biopharmaceuticals. Pressure-flow profiles are most commonly used to characterise these properties. There are a number of drawbacks with this method, including the potential need for several re-packs to achieve the desired packing quality, the impact of wall effects on experimental set up and the quantities of chromatography media and buffers required. To address these issues, we have developed a dynamic mechanical analysis (DMA) technique that characterises the mechanical properties of resins based on the viscoelasticity of a 1 ml sample of slurry. This technique was conducted on seven resins with varying degrees of mechanical robustness and the results were compared to pressure-flow test results on the same resins. Results show a strong correlation between the two techniques. The most mechanically robust resin (Capto Q) had a critical velocity 3.3 times higher than the weakest (Sepharose CL-4B), whilst the DMA technique showed Capto Q to have a slurry deformation rate 8.3 times lower than Sepharose CL-4B. To ascertain whether polymer structure is indicative of mechanical strength, scanning electron microscopy images were also used to study the structural properties of each resin. Results indicate that DMA can be used as a small volume, complementary technique for the mechanical characterisation of chromatography media.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1530, 29 December 2017, Pages 129-137
نویسندگان
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