کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7609406 1493397 2017 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Dispersive magnetic immunoaffinity extraction. Anatoxin-a determination
ترجمه فارسی عنوان
استخراج ایمنی مغناطیسی پراکنده. آناتوکسین- تعیین
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Specific monoclonal antibodies were coupled with magnetic Sepharose-based beads and used, for the first time. The methodology was applied to preconcentrate anatoxin-a from water and the later determination by ion mobility spectrometry (IMS). Dispersive magnetic immunoaffinity (d-MagIA) extraction methodology provided a limit of detection of 0.02 μg L−1 and a satisfactory precision with a relative standard deviation lower than 15%. Recoveries were evaluated at 0.5, 1.0 and 5.0 μg L−1 anatoxin-a with quantitative values from 91 to 115%. Additionally, isobaric interferences with phenylalanine were completely avoided by the use of the developed d-MagIA extraction coupled to IMS determinations.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1529, 22 December 2017, Pages 57-62
نویسندگان
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