کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7609437 | 1493398 | 2017 | 23 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Determination of γ-hydroxybutyrate in human urine samples by ion exclusion and ion exchange two-dimensional chromatography system
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Determination of γ-hydroxybutyrate in human urine samples by ion exclusion and ion exchange two-dimensional chromatography system Determination of γ-hydroxybutyrate in human urine samples by ion exclusion and ion exchange two-dimensional chromatography system](/preview/png/7609437.png)
چکیده انگلیسی
A two-dimensional ion chromatography system was developed for the determination of γ-hydroxybutyrate (GHB) in human urine samples. Ion exclusion chromatography was used in the first dimensional separation for elimination of urine matrices and detection of GHB above 10 mg Lâ1, ion exchange chromatography was used in the second dimensional separation via column-switching technique for detection of GHB above 0.08 mg Lâ1. Under the optimized chromatographic conditions, the ion exclusion and ion exchange chromatography separation system exhibited satisfactory repeatability (RSD < 3.1%, n = 6) and good linearity in the range of 50-1000 mg Lâ1 and 0.5-100 mg Lâ1, respectively. By this method, concentrations of GHB in the selected human urine samples were detected in the range of 0-1.57 mg Lâ1. The urine sample containing 0.89 mg Lâ1 GHB was selected to evaluate the accuracy; the spiked recoveries of GHB were 95.9-102.8%. The results showed that the two-dimensional ion chromatography system was convenient and practical for the determination of GHB in human urine samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1528, 15 December 2017, Pages 35-40
Journal: Journal of Chromatography A - Volume 1528, 15 December 2017, Pages 35-40
نویسندگان
Junwei Liu, Zhifen Deng, Zuoyi Zhu, Yong Wang, Guoqing Wang, Yu-an Sun, Yan Zhu,