کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7609512 | 1493400 | 2017 | 27 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development of immunosorbents coupled on-line to immobilized pepsin reactor and micro liquid chromatography-tandem mass spectrometry for analysis of butyrylcholinesterase in human plasma
ترجمه فارسی عنوان
توسعه ایمونواسروبنت ها به صورت مستقیم تا رآکتور پپسین مجهز شده و طیف سنج جرمی کروماتوگرافی مایع برای تجزیه و تحلیل بوتیریل کولین استراز در پلاسمای انسان
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Two pepsin-based IMERs were prepared and characterized in terms of grafting and digestion yields and were coupled on-line to microLC-MS/MS analysis. In addition, immunosorbents were prepared by covalent grafting of three anti-HuBuChE antibodies on CNBr-sepharose and epoxy-polymethacrylate supports and packed in precolumns. The best antibody grafting yields were obtained with sepharose-based supports, with grafting yields up to 98%. B2 18-5 monoclonal antibody grafted on sepharose led to the best immunosorbent, with HuBuChE recovery close to 100%. The immunosorbent was introduced upstream of the on-line digestion set-up and immunoextraction of HuBuChE was achieved in 14 min while digestion was performed in 20 min, allowing detection of the target nonapeptide in less than 1 h. The global recovery of the nonapeptide was higher than 42% using the best immunosorbent with a RSD value lower than 7% (n = 3). Finally, the limit of quantification evaluated in plasma sample was 2 fmol of nonapeptide. This value, corresponding to 0.5 fmol of HuBuChE tetramer, is well below the average amount of HuBuChE tetramer in 50 μL of plasma (590 fmol).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1526, 1 December 2017, Pages 70-81
Journal: Journal of Chromatography A - Volume 1526, 1 December 2017, Pages 70-81
نویسندگان
Maud Bonichon, Audrey Combès, Charlotte Desoubries, Anne Bossée, Valérie Pichon,