کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7609822 | 1493403 | 2017 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Assessment of intra-particle diffusion in hydrophilic interaction liquid chromatography and reversed-phase liquid chromatography under conditions of identical packing structure
ترجمه فارسی عنوان
ارزیابی انتشار داخل ذرات در کروماتوگرافی مایع کروماتوگرافی متقاطع هیدرولیکی و کروماتوگرافی مایع فاز برگشت پذیر در شرایط ساختار بسته بندی یکسان
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A recently developed stripping protocol to completely remove the stationary phase of reversed-phase liquid chromatography (RPLC) columns and turn them into hydrophilic interaction liquid chromatography (HILIC) columns with identical packing characteristics is used to study the underlying mechanisms of intra-particle diffusion in RPLC and HILIC. The protocol is applied to a column with a large geometrical volume (250 Ã 4.6 mm, 5 μm) to avoid extra-column effects and for compounds with a broad range in retention factors (k” from â¼0.6 to 8). Three types of behavior for the intra-particle diffusion (Dpart/Dm) in RPLC versus HILIC can be distinguished: for nearly unretained compounds (k” < 0.6), intra-particle diffusion in HILIC is larger than in RPLC; for compounds with intermediate retention behavior (k” â¼Â 0.9-1.2), intra-particle diffusion in HILIC and RPLC are similar; and for well retained compounds (k” > 1.8), intra-particle diffusion in RPLC is larger than in HILIC. To explain these observations, diffusion in the stationary phase (γsDs) and in the stagnant mobile phase in the mesopore zone (γmpDm) are deduced from experimentally determined values of the intra-particle diffusion, using models derived from the Effective Medium Theory. It is demonstrated that the larger intra-particle diffusion obtained for slightly retained compounds under HILIC conditions is caused by the higher mesopore diffusion in HILIC (γmp = 0.474 for HILIC versus 0.435 for RPLC), while the larger intra-particle diffusion obtained for strongly retained compounds under RPLC conditions can be related to the much higher stationary phase diffusion in RPLC (γsDs/Dm = 0.200 for RPLC versus 0.113 for HILIC).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1523, 10 November 2017, Pages 204-214
Journal: Journal of Chromatography A - Volume 1523, 10 November 2017, Pages 204-214
نویسندگان
Huiying Song, Gert Desmet, Deirdre Cabooter,