کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7609869 | 1493403 | 2017 | 33 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The versatility of heart-cutting and comprehensive two-dimensional liquid chromatography in monoclonal antibody clone selection
ترجمه فارسی عنوان
تطبیق پذیری کروماتوگرافی مایع کریستالیزاسیون دوجداره جامد در کلونهای آنتی بادی منوکلونال
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
In recent years, two-dimensional liquid chromatography (2D-LC) has seen an enormous evolution and one of the fields where it is being widely adopted is in the analysis of therapeutic monoclonal antibodies (mAbs). We here further add to the many flavours of this powerful technology. Workflows based on heart-cutting (LC-LC) and comprehensive (LCÂ ÃÂ LC) 2D-LC are described that allow to guide the clone selection process in mAb and biosimilar development. Combining Protein A affinity chromatography in the first dimension with size exclusion (SEC), cation exchange (CEX) or reversed-phase liquid chromatography-mass spectrometry (RPLC-MS) in the second dimension simultaneously allows to assess mAb titer and critical structural aspects such as aggregation, fragmentation, charge heterogeneity, molecular weight (MW), amino acid sequence and glycosylation. Complementing the LC-LC measurements at intact protein level with LCÂ ÃÂ LC based peptide mapping provides the necessary information to make clear decisions on which clones to take further into development.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1523, 10 November 2017, Pages 283-292
Journal: Journal of Chromatography A - Volume 1523, 10 November 2017, Pages 283-292
نویسندگان
Koen Sandra, Mieke Steenbeke, Isabel Vandenheede, Gerd Vanhoenacker, Pat Sandra,