کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7610469 1493497 2016 18 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Hybrid quadrupole-orbitrap mass spectrometry analysis with accurate-mass database and parallel reaction monitoring for high-throughput screening and quantification of multi-xenobiotics in honey
ترجمه فارسی عنوان
تجزیه و تحلیل اسپکترومتری جفتی چهارگروپول-اوربیتوگ با پایگاه داده دقیق جرم و مانیتورینگ موازی برای غربالگری با توان بالا و تعیین مقدار چندزبوبیوتیک در عسل
کلمات کلیدی
عسل، هیبرید چهاربعدی-اوربیتاپ، پایگاه داده جرم دقیق، نظارت موازی موازی، چند زبو بیوتیک،
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
This study reports a rapid, automated screening and quantification method for the determination of multi-xenobiotic residues in honey using ultra-high performance liquid chromatography-hybrid quadrupole-Orbitrap mass spectrometry (UHPLC-Q-Orbitrap) with a user-built accurate-mass database plus parallel reaction monitoring (PRM). The database contains multi-xenobiotic information including formulas, adduct types, theoretical exact mass and retention time, characteristic fragment ions, ion ratios, and mass accuracies. A simple sample preparation method was developed to reduce xenobiotic loss in the honey samples. The screening method was validated based on retention time deviation, mass accuracy via full scan-data-dependent MS/MS (full scan-ddMS2), multi-isotope ratio, characteristic ion ratio, sensitivity, and positive/negative switching performance between the spiked sample and corresponding standard solution. The quantification method based on the PRM mode is a promising new quantitative tool which we validated in terms of selectivity, linearity, recovery (accuracy), repeatability (precision), decision limit (CCα), detection capability (CCβ), matrix effects, and carry-over. The optimized methods proposed in this study enable the automated screening and quantification of 157 compounds in less than 15 min in honey. The results of this study, as they represent a convenient protocol for large-scale screening and quantification, also provide a research approach for analysis of various contaminants in other matrices.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1429, 15 January 2016, Pages 119-126
نویسندگان
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