کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7610888 | 1493501 | 2015 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Application of ultra-high performance supercritical fluid chromatography for the determination of carotenoids in dietary supplements
ترجمه فارسی عنوان
استفاده از کروماتوگرافی مایع فوق بحرانی فوق بحرانی برای تعیین کاروتنوئیدها در مکمل های غذایی
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
کاروتنوئیدها، کروماتوگرافی سیال فوق بحرانی فوق العاده بالا، مکمل غذایی،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A quick and simple ultra-high performance supercritical fluid chromatography-photodiode array detector method was developed and validated for the simultaneous determination of 9 carotenoids in dietary supplements. The influences of stationary phase, co-solvent, pressure, temperature and flow rate on the separation of carotenoids were evaluated. The separation of the carotenoids was carried out using an Acquity UPC2 HSS C18 SB column (150 mm Ã 3.0 mm, 1.8 μm) by gradient elution with carbon dioxide and a 1:2 (v:v) methanol/ethanol mixture. The column temperature was set to 35 °C and the backpressure was 15.2 MPa. Under these conditions, 9 carotenoids and the internal standard, β-apo-8â²-carotenal, were successfully separated within 10 min. The correlation coefficients (R2) of the calibration curves were all above 0.997, the limits of detection for the 9 carotenoids were in the range of 0.33-1.08 μg/mL, and the limits of quantification were in the range of 1.09-3.58 μg/mL. The mean recoveries were from 93.4% to 109.5% at different spiking levels, and the relative standard deviations were between 0.8% and 6.0%. This method was successfully applied to the determination of 9 carotenoids in commercial dietary supplements.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1425, 18 December 2015, Pages 287-292
Journal: Journal of Chromatography A - Volume 1425, 18 December 2015, Pages 287-292
نویسندگان
Bing Li, Haiyan Zhao, Jing Liu, Wei Liu, Sai Fan, Guohua Wu, Rong Zhao,