کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7610997 | 1493503 | 2015 | 23 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Qualitative and quantitative analysis of branches in dextran using high-performance anion exchange chromatography coupled to quadrupole time-of-flight mass spectrometry
ترجمه فارسی عنوان
تجزیه و تحلیل کمی و کیفی شاخه ها در دکستران با استفاده از کروماتوگرافی مبدل آنیونی با عملکرد بالا و همراه با طیف سنجی جرمی چهار ساعته پرواز
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Dextran, a family of natural polysaccharides, consists of an α (1â6) linked-glucose main (backbone) chain having a number of branches. The determination of the types and the quantities of branches in dextran is important in understanding its various biological roles. In this study, a hyphenated method using high-performance anion exchange chromatography (HPAEC) in parallel with pulsed amperometric detection (PAD) and mass spectrometry (MS) was applied to qualitative and quantitative analysis of dextran branches. A rotary cation-exchange cartridge array desalter was used for removal of salt from the HPAEC eluent making it MS compatible. MS and MS/MS were used to provide structural information on the enzymatically prepared dextran oligosaccharides. PAD provides quantitative data on the ratio of enzyme-resistant, branched dextran oligosaccharides. Both the types and degree of branching found in a variety of dextrans could be simultaneously determined online using this method.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1423, 4 December 2015, Pages 79-85
Journal: Journal of Chromatography A - Volume 1423, 4 December 2015, Pages 79-85
نویسندگان
Lin Yi, Yilan Ouyang, Xue Sun, Naiyu Xu, Robert J. Linhardt, Zhenqing Zhang,