کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7610997 1493503 2015 23 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Qualitative and quantitative analysis of branches in dextran using high-performance anion exchange chromatography coupled to quadrupole time-of-flight mass spectrometry
ترجمه فارسی عنوان
تجزیه و تحلیل کمی و کیفی شاخه ها در دکستران با استفاده از کروماتوگرافی مبدل آنیونی با عملکرد بالا و همراه با طیف سنجی جرمی چهار ساعته پرواز
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Dextran, a family of natural polysaccharides, consists of an α (1→6) linked-glucose main (backbone) chain having a number of branches. The determination of the types and the quantities of branches in dextran is important in understanding its various biological roles. In this study, a hyphenated method using high-performance anion exchange chromatography (HPAEC) in parallel with pulsed amperometric detection (PAD) and mass spectrometry (MS) was applied to qualitative and quantitative analysis of dextran branches. A rotary cation-exchange cartridge array desalter was used for removal of salt from the HPAEC eluent making it MS compatible. MS and MS/MS were used to provide structural information on the enzymatically prepared dextran oligosaccharides. PAD provides quantitative data on the ratio of enzyme-resistant, branched dextran oligosaccharides. Both the types and degree of branching found in a variety of dextrans could be simultaneously determined online using this method.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1423, 4 December 2015, Pages 79-85
نویسندگان
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