کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7611571 1493518 2015 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
An improved analytical strategy combining microextraction by packed sorbent combined with ultra high pressure liquid chromatography for the determination of fluoxetine, clomipramine and their active metabolites in human urine
ترجمه فارسی عنوان
استراتژی تحلیلی بهبود یافته ترکیب میکروکاکساسیون با جاذب بسته بندی شده همراه با کروماتوگرافی مایع با فشار بالا برای تعیین فلوکستین، کلومیپرامین و متابولیت های فعال آنها در ادرار انسان
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A powerful and sensitive method, by microextraction packed sorbent (MEPS), and ultra-high performance liquid chromatography (UHPLC) with a photodiode array (PDA) detection, is described for the determination of fluoxetine, clomipramine and their active metabolites in human urine samples. The MEPS variables, such as sample volume, pH, number of extraction cycles (draw-eject), and desorption conditions (solvent and solvent volume of elution) were optimized. The analysis were carried out using small sample volumes (500 μL) and in a short time period (5 min for the entire sample preparation step). Good linearity was obtained for all antidepressants with the correlation coefficients (R2) above 0.9965. The limits of detection (LOD) ranged from 0.068 to 0.087 μg mL−1. The recoveries were from 93% to 98%, with relative standard deviations less than 6%. The inter-day precision, expressed as the relative standard deviation, varied between 3.8% and 8.5% while the intra-day precision between 3.0% and 7.1%. In order to evaluate the proposed method for clinical use, the MEPS/UHPLC-PDA method was applied to analysis of urine samples from depressed patients.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1408, 21 August 2015, Pages 30-40
نویسندگان
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