Top-down and bottom-up lipidomic analysis of rabbit lipoproteins under different metabolic conditions using flow field-flow fractionation, nanoflow liquid chromatography and mass spectrometry

- Plasmic lipid analysis from rabbits under different metabolic conditions are performed.
- High cholesterol (HC) diet significantly alters the lipid profiles compared to factors of inflammation and dehydration.
- For the first time, top-down and bottom-up quantification of lipids from lipoproteins are directly compared.
- Top-down analysis with FlFFF-MS is found as useful for high speed quantitative analysis of lipoproteinic lipids.