کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7611930 1493543 2015 22 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Determination of short chain carboxylic acids in vegetable oils and fats using ion exclusion chromatography electrospray ionization mass spectrometry
ترجمه فارسی عنوان
تعیین اسید کربوکسیلیک زنجیره ای در روغن های گیاهی و چربی با استفاده از اسپکترومتر جرم یونیزاسیون الکترو اسپری کروماتوگرافی حذف یون
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A new method for quantification of short chain C1-C6 carboxylic acids in vegetable oils and fats by employing Liquid Chromatography Mass Spectrometry (LC-MS) has been developed. The method requires minor sample preparation and applies non-conventional Electrospray Ionization (ESI) liquid phase chemistry. Samples are first dissolved in chloroform and then extracted using water that has been spiked with stable isotope labeled internal standards that are used for signal normalization and absolute quantification of selected acids. The analytes are separated using Ion Exclusion Chromatography (IEC) and detected with Electrospray Ionization Mass Spectrometry (ESI-MS) as deprotonated molecules. Prior to ionization the eluent that contains hydrochloric acid is modified post-column to ensure good ionization efficiency of the analytes. The averaged within run precision and between run precision were generally lower than 8%. The accuracy was between 85 and 115% for most of the analytes. The Lower Limit of Quantification (LLOQ) ranged from 0.006 to 7 mg/kg. It is shown that this method offers good selectivity in cases where UV detection fails to produce reliable results.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1383, 27 February 2015, Pages 96-103
نویسندگان
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