کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7612366 1493556 2014 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Peptide imprinted receptors for the determination of the small cell lung cancer associated biomarker progastrin releasing peptide
ترجمه فارسی عنوان
گیرنده های ثبت شده پپتید برای تعیین سرطان سلول کوچک سلولی مرتبط با پپتید آزاد کننده پروگاستر بیومارکر
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Peptide imprinted polymers were developed for detection of progastrin releasing peptide (ProGRP); a low abundant blood based biomarker for small cell lung cancer. The polymers targeted the proteotypic nona-peptide sequence NLLGLIEAK and were used for selective enrichment of the proteotypic peptide prior to LCMS based quantification. Peptide imprinted polymers with the best affinity characteristics were first identified from a 96-polymer combinatorial library. The effects of functional monomers, crosslinker, porogen, and template on adsorption capacity and selectivity for NLLGLIEAK were investigated and optimized. Ultimately, a solid phase extraction method was developed for highly selective enrichment of the target peptide from tryptic digests.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1370, 28 November 2014, Pages 56-62
نویسندگان
, , , , , ,