کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7612374 1493556 2014 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Using contemporary liquid chromatography theory and technology to improve capillary gradient ion-exchange separations
ترجمه فارسی عنوان
با استفاده از تئوری کروماتوگرافی مایع معاصر و تکنولوژی برای بهبود بخشیدن به تبادل یونهای شیبانی مویرگی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
The gradient-performance limits of capillary ion chromatography have been assessed at maximum system pressure (34.5 MPa) using capillary columns packed with 4.1 μm macroporous anion-exchange particles coated with 65 nm positively-charged nanobeads. In analogy to the van-Deemter curve, the gradient performance was assessed applying different flow rates, while decreasing the gradient time inversely proportional to the increase in flow rate in order to maintain the same retention properties. The gradient kinetic-performance limits were determined at maximum system pressure, applying tG/t0 = 5, 10, and 20. In addition, the effect of retention on peak width was assessed in gradient mode for mono-, di-, and trivalent inorganic anions. The peak width of late-eluting ions can be significantly reduced by using concave gradient, resulting in better detection sensitivity. A signal enhancement factor of 8 was measured for a late-eluting ion when applying a concave instead of a linear gradient. For the analysis of a complex anion mixture, a coupled column with a total length of 1.05 m was operated at the kinetic-performance limit applying a linear 250 min gradient (tG/t0 = 10). The peak capacity varied between 200 and 380 depending on analyte retention, and hence on charge and size of the ion.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1370, 28 November 2014, Pages 63-69
نویسندگان
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