کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7613388 1493588 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Evaluation of capillary chromatographic supports for immobilized human purine nucleoside phosphorylase in frontal affinity chromatography studies
ترجمه فارسی عنوان
ارزیابی کروماتوگرافی های مویرگی برای فوروئلاز فوروئین فومیریمر در مطالعات کروماتوگرافی بالینی فرونتال
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
The aim of this work was to optimize the preparation of a capillary human purine nucleoside phosphorylase (HsPNP) immobilized enzyme reactor (IMER) for characterization and affinity screening studies of new inhibitors by frontal affinity chromatography coupled to mass spectrometry (FAC-MS). For this purpose two monolithic supports, a Chromolith Speed Rod (0.1 mm I.D. × 5 cm) and a methacrylate-based monolithic epoxy polymeric capillary column (0.25 mm I.D. × 5 cm) with epoxy reactive groups were considered and compared to an IMER previously developed using an open fused silica capillary. Each HsPNP-IMER was characterized in terms of catalytic activity using Inosine as standard substrate. Furthermore, they were also explored for affinity ranking experiments. Kd determination was carried out with the based fused silica HsPNP-IMER and the results are herein discussed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1338, 18 April 2014, Pages 77-84
نویسندگان
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