کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7613658 1493595 2014 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Ultra performance liquid chromatography analysis to study the changes in the carotenoid profile of commercial monovarietal fruit juices
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Ultra performance liquid chromatography analysis to study the changes in the carotenoid profile of commercial monovarietal fruit juices
چکیده انگلیسی
We have developed an analytical method that allows the simultaneous determination of epoxycarotenoids, hydroxycarotenoids and carotenes in monovarietal fresh homemade and industrially processed fruit products. Analyses were carried out using ultra performance liquid chromatography (UPLC). The extraction method was optimized using methanol as the first extraction solvent for lyophilized samples followed by a saponification step. Recoveries ranged between 75% and 104% depending on the compound. Repeatability was better than 10% for all compounds (%RSD, n = 3). The chromatographic analysis takes less than 17 min. In this short period, up to 27 carotenoids were identified in apple, peach and pear products. The developed method allowed us to differentiate juice from six varieties of apple by their carotenoid profile. Moreover, the methodology allows us to differentiate the carotenoid profiles from commercial juices and homemade fresh peach and pear juices, as well as to study the rearrangements of 5,6- to 5,8-epoxycarotenoids.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1331, 28 February 2014, Pages 90-99
نویسندگان
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