کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7614016 1493610 2013 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Ultrathin-yttrium phosphate-shelled polyacrylate-ferriferrous oxide magnetic microspheres for rapid and selective enrichment of phosphopeptides
ترجمه فارسی عنوان
میکروسکپ های مغناطیسی اکسید فرایفری پتاسیم فسفات اولتراتین و یتیم فسفات برای غنی سازی سریع و انتخابی فسفوپپتید
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Rapid and selective enrichment of phosphopeptides from complex biological samples is essential and challenging in phosphorylated proteomics. We present the direct growth of the ultrathin YPO4 shell on the surface of polyacrylate capped secondary Fe3O4 microspheres (PA-Fe3O4@YPO4) for the rapid and selective trapping phosphopeptides from complex samples. The prepared PA-Fe3O4@YPO4 could be rapidly harvested in the presence of an applied magnetic field and easily re-dispersed in solutions after removing the external magnet. The ultrathin YPO4 shell on super-hydrophilic PA-Fe3O4 has the advantages of fast adsorption/desorption dynamics and low non-specific adsorption, thus trapping of phosphopeptides from the tryptic digests mixture of β-casein/BSA with molar ratio of 1/300 is achieved in 20 s adsorption/desorption time. Two phosphopeptides can still be detected with a signal to noise ratio (S/N) over 3 when the amount of β-casein was as low as 8 fmol.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1316, 5 November 2013, Pages 62-68
نویسندگان
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