کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7614203 | 1493621 | 2013 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A rapid method for the determination of perfluoroalkyl substances including structural isomers of perfluorooctane sulfonic acid in human serum using 96-well plates and column-switching ultra-high performance liquid chromatography tandem mass spectrometry
ترجمه فارسی عنوان
یک روش سریع برای تعیین مواد پرفروفیالکلیک از جمله ایزومرهای ساختاری پرفیولوروکتان سولفونیک اسید در سرم انسان با استفاده از صفحات 96 اینچ و کروماتوگرافی مایع با ستون سوئیچ ستون، طیف سنجی جرمی
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
To facilitate high-throughput analysis suitable for large epidemiological studies we developed an automated column-switching ultra-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for determination of perfluorocarboxylic acids (PFCAs; C5, C6, C7, C8, C9, C10, C11, C12, and C13), perfluoroalkyl sulfonic acids (PFSAs; C4, C6, C8, and C10), perfluorooctane sulfonamide (PFOSA), and five groups of structural perfluorooctane sulfonic acid (PFOS) isomers in human serum or plasma. The analytical procedure involves rapid protein precipitation using 96-well plates followed by an automated sample clean-up using an on-line trap column removing many potentially interfering sample components while through the mobile phase gradient the target analytes are eluted onto the analytical column for further separation and subsequent mass detection. The method was linear (R2 < 0.995) at concentrations ranging from 0.01 to 60 ng mLâ1 with method detection limits ranging between 0.01 and 0.17 ng mLâ1 depending on the analyte. The developed method was precise, with repeatability (n = 7) and reproducibility (n = 103) coefficients of variation between 2% and 20% for most compounds including PFOS (2% and 8%) and its structural isomers (2-6% and 4-8%). The method was in conformity with a standard reference material. The column-switching HPLC-MS/MS method has been successfully applied for the determination of perfluoroalkyl substances including structural PFOS isomers in human plasma from an epidemiological study.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1305, 30 August 2013, Pages 164-170
Journal: Journal of Chromatography A - Volume 1305, 30 August 2013, Pages 164-170
نویسندگان
Samira Salihovic, Anna Kärrman, Gunilla Lindström, P. Monica Lind, Lars Lind, Bert van Bavel,