Multiclass mycotoxin analysis in Silybum marianum by ultra high performance liquid chromatography-tandem mass spectrometry using a procedure based on QuEChERS and dispersive liquid-liquid microextraction

► A UHPLC-MS/MS method is proposed for determination of 15 mycotoxins in milk thistle. ► Sample treatment is based on QuEChERS extraction and DLLME clean-up. ► LOQs are below maximum limits allowed by EU in foods and botanicals. ► RSDs were <10% and recoveries were >60%, except for ZON in seed and CIT in extract. ► The method could be good alternative for multi-mycotoxin determination in botanicals.