Characterization and quantification of racemic and meso-ethylenediamine-N,N′-bis(2-hydroxy-5-sulfophenylacetic) acid/iron (III) by ion-pair ultra-high performance liquid chromatography coupled with diode array detector and electrospray tandem mass spect

► Synthesis and quantification of EDDHSA ligand. ► Set up of the UHPLC-Vis-ESI/MS method for rac and meso-EDDHSA/Fe determination. ► the stereochemistries of the two EDDHSA/Fe isomers were assigned. ► Mass fragmentation patterns of rac and meso-EDDHSA/Fe were determined. ► New insights on ion-pair chromatography were provided.