Discovery of candidate phospholipid biomarkers in human lipoproteins with coronary artery disease by flow field-flow fractionation and nanoflow liquid chromatography-tandem mass spectrometry

► HDL and LDL fractions of plasma were separated by MxHF5. ► Significant changes in lipids in each lipoprotein were observed between controls and patients. ► A total of eleven lipid species were selected as candidate biomarkers of CAD.