Comparison of the fast gradient performance of new prototype silica monolithic columns and columns packed with fully porous and core-shell particles

► We used new prototypes of narrow-bore monolithic columns for gradient elution analyses. ► A commercial check-out sample was analyzed. ► So was a non-polar extract of cultures of Botryococcus braunii, Showa race B. ► The performance of three monolithic columns were compared to those of commercial packed columns. ► The performance of the second generation monolithic columns compare well to those of packed columns.