کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7614795 | 1493976 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Quantitative analysis of dextran in rat plasma using Q-Orbitrap mass spectrometry based on all ion fragmentation strategy
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کلمات کلیدی
DEXLLOQSECMRMHCDNCEAIF - آیفونinternal standard - استاندارد داخلیnormalized collision energy - انرژی برخورد عادیDextran - دکسترانpharmacokinetic - فارماکوکینتیکmultiple reaction monitoring - نظارت چندگانه چندگانهSize exclusion chromatography - کروماتوگرافی اندازهای طردیquality control - کنترل کیفیت
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Dextran is a biocompatible glucose-based polymer that is widely used clinically as a plasma volume expander, anti-thrombotic agent, macromolecular carrier, peripheral blood flow enhancer, and artificial tears promoter. Because dextran has polydisperse molecular weights and tends to produce innumerable multi-charged precursor ions in the ion source, it is difficult to analysis this polymer using conventional liquid chromatography mass spectrometry. In this assay, all ion fragmentation strategy is used to solve this problem by allowing all dextran precursor ions generated in the ion source to enter the collision cell. Then serial dextran-specific fragments can be effectively generated from all precursor ions by higher energy collision dissociation and scanned by Orbitrap detector. These high resolution fragment ions provide high specificity and sensitivity for the quantitation of dextran in rat plasma. Here, we report a new quantitative method using size exclusion chromatography combined with Q Exactive mass spectrometry based on an all ion fragmentation strategy. Assay validation showed that this method is linear over the concentration range from 3 to 300â¯Î¼g/mL. Our approach has been successfully applied to a pharmacokinetic study of dextran in rat and will promote the development of studies with other polysaccharides.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 1095, 15 September 2018, Pages 24-31
Journal: Journal of Chromatography B - Volume 1095, 15 September 2018, Pages 24-31
نویسندگان
Hao Wang, Honglei Chen, Jie Geng, Yi Zheng, Zhongyu Zhang, Lin Sun, Guihua Tai, Yifa Zhou,