کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7614815 | 1493977 | 2018 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Rapid analysis of neomycin in cochlear perilymph of guinea pigs using disposable SPE cartridges and high performance liquid chromatography-tandem mass spectrometry
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کلمات کلیدی
LLOQMRMABRHFBAHPLC-MS/MS - HPLC-MS / MSRWM - RWMheptafluorobutyric acid - اسید heptafluorobutyricLOD یا Limit of detection - حد تشخیصCoefficient of Variation - ضریب تغییرRound Window Membrane - غشای پنجره گردlimit of detection - محدودیت تشخیصlower limit of quantitation - محدودیت پایین کمیتNeomycin - نومیدینauditory brainstem response - پاسخ شنوایی مغزPerilymph - پریلیمفquality control - کنترل کیفیت
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Irreversible hearing loss induced by aminoglycoside in human through local or systemic administration route negatively impacts quality of life. The aim of this work was to develop and validate an analytical method suitable for the detection and quantification of neomycin in cochlear perilymph of guinea pig after local application. The SupelMIP SPE column was used for the pre-treatment of matrix. Chromatographic separation was conducted by a reversed phase ODS column (100â¯Ãâ¯2.1â¯mm, 3â¯Î¼m) at 40â¯Â°C in gradient mode with 0.2â° (v/v) HFBA in water and 0.2â° (v/v) HFBA in acetonitrile as mobile phase, at a flow rate of 0.30â¯mL/min, with retention time of 3.50 and 3.62â¯min for internal standard tobramycin and analyte neomycin, respectively. The MS was performed with positive ionization mode, with data acquisition in Multi Reaction Monitor (MRM) mode. This method was proved to be specific, accurate (97.1-115% of nominal values) and precise (CV%â¯<â¯15%). Calibration curves for matrix matched standard of neomycin ranged from 1.25 to 200â¯Î¼g/mL, with LOD and LLOQ of 0.625 and 1.25â¯Î¼g/mL in blank matrix. The matrix effect was corrected to (â0.1) - 1.33 by adding internal standard. The relative SPE recovery values were â¥98.9% in low, medium and high QC samples. Neomycin in matrix proved to be stable under room temperature - and â20â¯Â°C, or under three freeze-thawing cycles, or under processing as well. Finally, the proposed method was successfully applied to a toxicokinetics study of neomycin in perilymph after round window membrane (RWM) administration, which was in accordance with threshold shift of auditory brainstem response (ABR) test related to hearing loss.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volumes 1093â1094, 1 September 2018, Pages 52-59
Journal: Journal of Chromatography B - Volumes 1093â1094, 1 September 2018, Pages 52-59
نویسندگان
Mian Zu, Jinan Jiang, Hui Zhao, Shili Zhang, Yan Yan, Shiwei Qiu, Shuolong Yuan, Jiawei Han, Yue Zhang, Weiwei Guo, Shiming Yang,