کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7614980 | 1493978 | 2018 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Simultaneous quantification of straight-chain and branched-chain short chain fatty acids by gas chromatography mass spectrometry
ترجمه فارسی عنوان
اندازه گیری همزمان اسیدهای چرب زنجیره کوتاه زنجیره ای بدون زنجیره و زنجیره ای با اسپکترومتری جرمی کروماتوگرافی گاز
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Biomedical research in areas such as metabolic disorders, neuromodulatory, and immunomodulatory conditions involves lipid metabolism and demands a reliable and inexpensive method for quantification of short chain fatty acids (SCFAs). We report a GC-MS method for analysis of all straight-chain and branched-chain SCFAs using pentafluorobenzyl bromide (PFBBr) as derivatization reagent. We optimized the derivatization and GC-MS conditions using a mixture containing all eight SCFA standards, i.e., five straight-chain and three branched-chain SCFAs. The optimal derivatization conditions were derivatization time 90â¯min, temperature 60â¯Â°C, pHâ¯7, and (CH3)2CO:H2O ratio 2:1 (v:v). Comparing the performance of different GC column configurations, a 30â¯m DB-225ms hyphenated with a 30â¯m DB-5ms column in tandem showed the best separation of SCFAs. Using the optimized experiment conditions, we simultaneously detected all SCFAs with much improved detection limit, 0.244-0.977â¯Î¼M. We further applied the developed method to measure the SCFAs in mouse feces and all SCFAs were successfully quantified. The recovery rates of the eight SCFAs ranged from 55.7% to 97.9%.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 1092, 15 August 2018, Pages 359-367
Journal: Journal of Chromatography B - Volume 1092, 15 August 2018, Pages 359-367
نویسندگان
Liqing He, Md Aminul Islam Prodhan, Fang Yuan, Xinmin Yin, Pawel K. Lorkiewicz, Xiaoli Wei, Wenke Feng, Craig McClain, Xiang Zhang,