کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7615005 | 1493978 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
An effective and high-throughput analytical methodology for pesticide screening in human urine by disposable pipette extraction and gas chromatography - mass spectrometry
ترجمه فارسی عنوان
یک روش تحلیلی موثر و با توان بالا برای غربالگری آفت کش ها در ادرار انسان با استفاده از استخراج پیپت یکبار مصرف و کروماتوگرافی گاز - طیف سنج جرمی
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
In this paper, the use of disposable pipette extraction (DPX) for the determination of pesticides in human urine in possible cases of poisoning is proposed for the first time. The pesticides studied were oxamyl, propoxur, carbofuran, 3âhydroxycarbofuran, carbaryl, methiocarb, terbufos, parathion methyl, malathion, chlorpyrifos and endosulfan. The pipette tip used for the extraction of these compounds was commercially acquired. It has a capacity of 5â¯mL and contains 20â¯mg of sorbent material (styrene-divinylbenzene). The optimization of the main parameters that can influence the extraction efficiency of this sample preparation technique was performed with univariate and multivariate approaches. The analytes were separated and identified by gas chromatography coupled to mass spectrometry (GC-MS). The optimal extraction conditions were 5 extraction cycles of 30â¯s and 5 desorption cycles of 15â¯s with 250â¯Î¼L of ethyl acetate. Elution of the extract was performed in a vial containing 100â¯mg of anhydrous sodium sulfate. The method developed was validated, providing correlation coefficients higher than 0.9955 for all analytes, limits of detection (LOD) of 0.76 to 1.52â¯Î¼gâ¯Lâ1, limits of quantification (LOQ) of 2.5 to 5.0â¯Î¼gâ¯Lâ1, relative recoveries of 63 to 118%, intra-day precision of 0.7 to 15.3% and inter-day precision of 4.9 to 13.1%. An effective and rapid method for the analysis of human urine for the identification of possible cases of poisoning by pesticides was successful developed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 1092, 15 August 2018, Pages 459-465
Journal: Journal of Chromatography B - Volume 1092, 15 August 2018, Pages 459-465
نویسندگان
Anderson Luiz Oenning, Josias Merib, Eduardo Carasek,