کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7615050 | 1493982 | 2018 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development of a hematocrit-insensitive device to collect accurate volumes of dried blood spots without specialized skills for measuring clozapine and its metabolites as model analytes
ترجمه فارسی عنوان
توسعه یک دستگاه غیر حساس به هماتوکریت برای جمع آوری حجم دقیق لکه های خون خشک شده بدون مهارت های تخصصی برای اندازه گیری کلوزاپین و متابولیت های آن به عنوان آنالیتورهای مدل
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کلمات کلیدی
LLOQ%RSDN-desmethylclozapineHPLC-CDLOD یا Limit of detection - حد تشخیصlower limit of quantification - حد پایین کمیت سنجیSelf-sampling - خود نمونه برداریpercentage coefficient of variation - درصد ضریب تغییراتlimit of detection - محدودیت تشخیصDried blood spot - نقطه خالی خشکhematocrit - هماتوکریتHPLC - کروماتوگرافی مایعی کاراclozapine - کلوزاپین
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Dried blood spots have been used as alternatives to traditional plasma and serum samples. We have now developed new devices, named volumetric absorptive paper disc (VAPD) and mini-disc (VAPDmini), to collect accurate volumes of dried blood spots in a simple manner and without the need for additional instruments. VAPD consists of a filter paper disc and a filter paper sheet with holes slightly larger than the disc. The disc is fixed in one such hole without direct contact with the filter sheet. VAPDmini is a scaled-down version of the same device. When several drops of whole blood are applied, the disc becomes saturated and any excess sample is absorbed by the surrounding filter sheet. Accuracy and precision of sampling were assessed by determining the levels of clozapine and its metabolites as target analytes by liquid-liquid extraction and high-performance liquid chromatography with coulometric detection. In addition, differences in analyte recovery were within ±15% for all analytes in samples with 30-60% hematocrit, suggesting that VAPD and VAPDmini are insensitive to hematocrit for the analytes tested. The devices were also validated for analyte concentrations in the range 50-1000â¯ng/mL, and the limit of detection and lower limit of quantification were 5-17â¯ng/mL and 15-51â¯ng/mL, respectively. Intra- and inter-day precision ranged from 3% to 13%, whereas accuracy ranged from a â14% to 12% bias. Analytes were stable in the devices for at least 2â¯weeks at room temperature. Collectively, these results indicate that sampling using VAPD and VAPDmini is comparable to conventional hole punch sampling of entire dried blood spots, even for samples obtained from patients treated with clozapine. Importantly, the devices were also found to be suitable for sample self-collection.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volumes 1087â1088, 15 June 2018, Pages 70-79
Journal: Journal of Chromatography B - Volumes 1087â1088, 15 June 2018, Pages 70-79
نویسندگان
Tatsuo Nakahara, Natsumi Otani, Takefumi Ueno, Kijiro Hashimoto,