کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7615078 | 1493983 | 2018 | 32 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Investigation of anti-Hepatitis C virus, sofosbuvir and daclatasvir, in pure form, human plasma and human urine using micellar monolithic HPLC-UV method and application to pharmacokinetic study
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Simultaneous determination of sofosbuvir (SOF), and daclatasvir (DAC) in their dosage forms, human urine and human plasma using simple and rapid micellar high performance liquid chromatographic method coupled with UV detection (HPLC-UV) had been developed and validated. These drugs are described as co-administered for treatment of Hepatitis C virus (HCV). HCV is the cause of Hepatitis C and some cancers such as liver cancer (hepatocellular carcinoma) and lymphomas in humans. Separation and quantitation were carried out on anonyx⢠C8 monolithic (100â¯Ãâ¯4.6â¯mm (i.d.) analytical column maintained at 25â¯Â°C. The mobile phase consisted of 0.1â¯M sodium dodecyl sulfate (SDS) solution containing 20% (V/V) n-propanolol and 0.3% (V/V) triethylamine and pH was adjusted to 6.5 using 0.02â¯M phosphoric acid, respectively. The retention times of SOF and DAC were 4.8â¯min, and 6.5â¯min, respectively. Measurements were made at flow rate of 0.5â¯mL/min with injection volume of 20â¯Î¼L and ultraviolet (UV) detection at 226â¯nm. Linearity of SOF and DAC was obtained over concentration ranges of 50-400, and 40-400â¯ng/mL, respectively in pure form, 60-300 and 50-300â¯ng/mL, respectively for human plasma and over 50-400, and 40-400â¯ng/mL, respectively for human urine with correlation coefficient >0.999. The proposed method demonstrated excellent intra- and inter-day precision and accuracy. The suggested method was applied for determination of the drugs in pure, dosage form, and in real human plasma, real human urine and drug-dissolution test of their tablets. The obtained results have been statistically compared to reported method to give a conclusion that there is no significant differences.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 1086, 1 June 2018, Pages 73-81
Journal: Journal of Chromatography B - Volume 1086, 1 June 2018, Pages 73-81
نویسندگان
Dalia W. Zidan, Wafaa S. Hassan, Manal S. Elmasry, Abdalla A. Shalaby,