کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7615161 | 1493985 | 2018 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Quantitation of peptides from non-invasive skin tapings using isotope dilution and tandem mass spectrometry
ترجمه فارسی عنوان
شمارش پپتیدها از تکه های پوست غیر تهاجمی با استفاده از ریزش ایزوتوپ و طیف سنجی جرم دو طرفه
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کلمات کلیدی
چسباندن پوست، طیف سنجی جرمی، پروتئومیکس، درماتیت آتوپیک، کمترین، رقیق ایزوتوپ،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Previous work from our laboratories utilized a novel skin taping method and mass spectrometry-based proteomics to discover clinical biomarkers of skin conditions; these included atopic dermatitis, Staphylococcus aureus colonization, and eczema herpeticum. While suitable for discovery purposes, semi-quantitative proteomics is generally time-consuming and expensive. Furthermore, depending on the method used, discovery-based proteomics can result in high variation and inadequate sensitivity to detect low abundant peptides. Therefore, we strove to develop a rapid, sensitive, and reproducible method to quantitate disease-related proteins from skin tapings. We utilized isotopically-labeled peptides and tandem mass spectrometry to obtain absolute quantitation values on 14 peptides from 7 proteins; these proteins had shown previous importance in skin disease. The method demonstrated good reproducibility, dynamic range, and linearity (R2â¯>â¯0.993) when nâ¯=â¯3 standards were analyzed across 0.05-2.5â¯pmol. The method was used to determine if differences exist between skin proteins in a small group of atopic versus non-atopic individuals (nâ¯=â¯12). While only minimal differences were found, peptides were detected in all samples and exhibited good correlation between peptides for 5 of the 7 proteins (R2â¯=â¯0.71-0.98). This method can be applied to larger cohorts to further establish the relationships of these proteins to skin disease.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 1084, 1 May 2018, Pages 132-140
Journal: Journal of Chromatography B - Volume 1084, 1 May 2018, Pages 132-140
نویسندگان
Nichole Reisdorph, Michael Armstrong, Roger Powell, Kevin Quinn, Kevin Legg, Donald Leung, Rick Reisdorph,