کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7615274 | 1493986 | 2018 | 22 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Factors affecting the separation performance of proteins in capillary electrophoresis
ترجمه فارسی عنوان
عوامل موثر بر عملکرد جداسازی پروتئین در الکتروفورز مویرگی
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
الکتروفورز مویرگی، پروتئین، فلورسانس،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Capillary electrophoresis (CE) is an effective tool for protein separation and analysis. Compared with capillary gel electrophoresis (CGE), non-gel sieving capillary electrophoresis (NGSCE) processes the superiority on operation, repeatability and automaticity. Herein, we investigated the effect of polymer molecular weight and concentration, electric field strength, and the effective length of the capillary on the separation performance of proteins, and find that (1) polymer with high molecular weight and concentration favors the separation of proteins, although concentrated polymer hinders its injection into the channel of the capillary due to its high viscosity. (2) The resolution between the adjacent proteins decreases with the increase of electric field strength. (3) When the effective length of the capillary is long, the separation performance improves at the cost of separation time. (4) 1.4% (w/v) hydroxyethyl cellulose (HEC), 100â¯V/cm voltage and 12â¯cm effective length offers the best separation for the proteins with molecular weight from 14,400â¯Da to 97,400â¯Da. Finally, we employed the optimal electrophoretic conditions to resolve Lysozyme, Ovalbumin, BSA and their mixtures, and found that they were baseline resolved within 15â¯min.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 1083, 15 April 2018, Pages 63-67
Journal: Journal of Chromatography B - Volume 1083, 15 April 2018, Pages 63-67
نویسندگان
Yueping Zhu, Zhenqing Li, Ping Wang, Lisong Shen, Dawei Zhang, Yoshinori Yamaguchi,