کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7615387 | 1493991 | 2018 | 30 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
RP-HPLC method for simultaneous estimation of vigabatrin, gamma-aminobutyric acid and taurine in biological samples
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کلمات کلیدی
LLOQNDARP-HPLCvigabatrinUS FDA - FDA ایالات متحدهUnited States Food and Drug Administration - اداره غذا و داروی ایالات متحدهinternal standard - استاندارد داخلیGamma aminobutyric acid - اسید گاما آمینو بوتیریکMethod validation - اعتبار سنجی روشTaurine - تائورینlower limit of quantification - حد پایین کمیت سنجیreverse phase-high performance liquid chromatography - کروماتوگرافی مایع فاز با عملکرد بالا معکوسGABA - گابا
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Vigabatrin is used as first line drug in treatment of infantile spasms for its potential benefit overweighing risk of causing permanent peripheral visual field defects and retinal damage. Chronic administration of vigabatrin in rats has demonstrated these ocular events are result of GABA accumulation and depletion of taurine levels in retinal tissues. In vigabatrin clinical studies taurine plasma level is considered as biomarker for studying structure and function of retina. The analytical method is essential to monitor taurine levels along with vigabatrin and GABA. A RP-HPLC method has been developed and validated for simultaneous estimation of vigabatrin, GABA and taurine using surrogate matrix. Analytes were extracted from human plasma, rat plasma, retina and brain by simple protein precipitation method and derivatized by naphthalene 2, 3âdicarboxaldehyde to produce stable fluorescent active isoindole derivatives. The chromatographic analysis was performed on Zorbax Eclipse AAA column using gradient elution profile and eluent was monitored using fluorescence detector. A linear plot of calibration curve was observed in concentration range of 64.6 to 6458, 51.5 to 5150 and 62.5 to 6258â¯ng/mL for vigabatrin, GABA and taurine, respectively with r2â¯â¥â¯0.997 for all analytes. The method was successfully applied for estimating levels of vigabatrin and its modulator effect on GABA and taurine levels in rat plasma, brain and retinal tissue. This RP-HPLC method can be applied in clinical and preclinical studies to explore the effect of taurine deficiency and to investigate novel approaches for alleviating vigabatrin induced ocular toxicity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 1076, 15 February 2018, Pages 44-53
Journal: Journal of Chromatography B - Volume 1076, 15 February 2018, Pages 44-53
نویسندگان
Anitha Police, Vijay Kumar Shankar, S. Narasimha Murthy,