کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7617657 | 1494079 | 2014 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Liquid chromatography-mass spectrometry measurement of leukotrienes in asthma and other respiratory diseases
ترجمه فارسی عنوان
اندازه گیری کروماتوگرافی مایع اسپکترومتری از لکو تریژن ها در آسم و سایر بیماری های تنفسی
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کلمات کلیدی
التهاب هواپیما، آسم، بیومارکرها، مایع متراکم نفس لوکوترین ها، طیف سنجی جرم کروماتوگرافی مایع، داروهای تنفسی،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Leukotrienes (LTs), including cysteinyl-LTs (LTC4, LTD4 and LTE4) and LTB4, are potent inflammatory lipid mediators which have been involved in the pathophysiology of respiratory diseases. LC-MS/MS techniques for measuring LT concentrations in sputum supernatants, serum, urine and exhaled breath condensate (EBC) have been developed. In asthmatic adults, reported LTB4 and LTE4 concentrations in sputum range from 79 to 7220Â pg/ml and from 11.9 to 891Â pg/ml, respectively. Data on sputum LT concentrations in healthy subjects are not available. In EBC, reported LTE4 concentrations range from 38 to 126Â pg/ml (95% CI) in adult asthma patients and from 34 to 48Â pg/ml in healthy subjects. LTB4 concentrations in EBC range from 175 to 315Â pg/ml (interquartile range) in asthmatic children, and from 25 to 245Â pg/ml in healthy children. Enabling an accurate quantitative assessment of LTs in biological fluids, LC-MS/MS techniques provide a valuable tool for exploring the pathophysiological role of LTs in respiratory disease and might be useful for assessing the effects of therapeutic intervention. This review presents the analytical aspects of the LC-MS/MS techniques for measuring LT concentrations in biological fluids and discusses their potential utility for the assessment of airway inflammation and monitoring of pharmacological treatment in patients with asthma phenotypes and other respiratory diseases.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 964, 1 August 2014, Pages 12-25
Journal: Journal of Chromatography B - Volume 964, 1 August 2014, Pages 12-25
نویسندگان
Paolo Montuschi, Giuseppe Santini, Salvatore Valente, Chiara Mondino, Francesco Macagno, Paola Cattani, Gina Zini, Nadia Mores,