کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7617687 | 1494082 | 2014 | 26 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Determination of homocysteine thiolactone in urine by field amplified sample injection and sweeping MEKC method with UV detection
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Determination of homocysteine thiolactone in urine by field amplified sample injection and sweeping MEKC method with UV detection Determination of homocysteine thiolactone in urine by field amplified sample injection and sweeping MEKC method with UV detection](/preview/png/7617687.png)
چکیده انگلیسی
Homocysteine thiolactone (Hcy-thiolactone), an intramolecular thioester, easily acylates free-amino groups in proteins, which impairs or alters the protein's biological function. Here, we describe new capillary electrophoresis assay for the determination of Hcy-thiolactone in human urine based on a field amplified sample injection and sweeping MEKC with UV detection. The two steps procedure relies on sample liquid-liquid extraction followed by CE separation and UV detection at 240 nm. The Hcy-thiolactone standard added to the urine before the extraction step shows that the response of the detector is linear within the range studied, from 0.1 to 1 μmol Lâ1 urine. The intra- and interday precision and recovery were 3.2-14.4% (average 5.1% and 9.3%) and 92.5-112.6% (average 99.8% and 99.1%), respectively. The lower limit of quantification was 0.09 nmol Hcy-thiolactone in 1 mL of urine. The proposed method was applied for the analysis of 15 urine samples donated by apparently healthy volunteers. The average concentration of the analyte was 0.170 ± 0.029 μmol Lâ1.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 961, 15 June 2014, Pages 36-41
Journal: Journal of Chromatography B - Volume 961, 15 June 2014, Pages 36-41
نویسندگان
Paulina Furmaniak, PaweÅ Kubalczyk, RafaÅ GÅowacki,