کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7617733 | 1494083 | 2014 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Application of optimized dispersive liquid-liquid microextraction for determination of melatonin by HPLC-UV in plasma samples
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Melatonin (N-acetyl-3-(2-aminoethyl)-5-methoxyindole) is biologically active as a neurohormone and antioxidant agent. The optimized dispersive liquid-liquid microextraction (DLLME) followed by high-performance liquid chromatography-ultra violet detection (HPLC-UV) was used for the analysis of melatonin in human plasma. Influence variables such as volume of extracting (carbon tetrachloride: CCl4) and dispersing solvents (acetonitrile: ACN), pH and ionic strength, extraction time and centrifugation time were screened in a 26â2 fractional factorial design (FFD) and then the significant variables were optimized by using a central composite design (CCD). At optimum conditions values of variables set as pH 6.0, 1.5 mL ACN, 140 μL CCl4, 1.0 min extraction time and 3.0 min centrifugation at 4500 rpm. At optimum conditions method has linear response over 2.0-500.0 ng mLâ1 with detection limit of 0.5 ng mLâ1 with relative standard deviations (RSDs) less than 5.0%. The values of intra-day and inter-day RSD were 4.3% and 8.5%, respectively. The method was applied successfully for the analysis of melatonin in plasma sample.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 960, 1 June 2014, Pages 1-7
Journal: Journal of Chromatography B - Volume 960, 1 June 2014, Pages 1-7
نویسندگان
M.S. Talebianpoor, S. Khodadoust, A. Rozbehi, M. Akbartabar Toori, M. Zoladl, M. Ghaedi, R. Mohammadi, A.S. Hosseinzadeh,