کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7617752 | 1494083 | 2014 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development and validation of LC-MS/MS method for quantitative determination of (â)-securinine in mouse plasma
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
(â)-Securinine (SE) is a major alkaloid found in plant Securinega suffruticosa, which has a wide range of pharmacological activities including anticancer, anti-parasitic and central nervous system stimulating effects, etc. To aid the pharmacological study of SE, we developed an LC-MS/MS method for quantitative determination of SE in mouse plasma. In this method, plasma samples were first prepared with salting-out assisted liquid-liquid extraction using cold acetonitrile (â20 °C) and 2.00 M ammonium acetate. Separation of SE and the internal standard (IS) from sample matrix was achieved on a Gemini Nx C18 column using 40% acetonitrile and 60% 10.0 mM ammonium acetate at a flow rate of 0.200 mL minâ1. Quantification of SE was accomplished with positive electrospray ionization tandem mass spectrometry using mass transitions m/z 218.1 â 84.1 for SE, and m/z 204.1 â 70.2 for the IS. This method has a lower limit of quantitation (LLOQ) of 0.600 ng mLâ1 and a linear calibration range up to 600 ng mLâ1 in mouse plasma. The intra- and inter-run accuracy (%RE) and precision (%CV) were â¤Â±6% and 6%, respectively. The IS normalized matrix factors from six lots of plasma matrices ranged 0.92-1.07, and the recoveries of plasma SE were 99-109%. The validated method has been applied to the measurement of SE in plasma samples of a mouse study.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 960, 1 June 2014, Pages 19-26
Journal: Journal of Chromatography B - Volume 960, 1 June 2014, Pages 19-26
نویسندگان
Simuli L. Wabuyele, David Wald, Yan Xu,