کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7618132 | 1494101 | 2013 | 27 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A simple and robust high-performance liquid chromatography coupled to a diode-array detector method for the analysis of genistein in mouse tissues
ترجمه فارسی عنوان
یک کروماتوگرافی مایع با کارایی با کارایی ساده و قوی همراه با یک روش تشخیص آرایه دیود برای تجزیه ژن هایستین در بافت موش
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
A simple liquid-liquid extraction procedure and quantification by high-performance liquid chromatography (HPLC) method coupled to a diode-array detector (DAD) of genistein (GEN) was developed in various mouse biological matrices. 7-ethoxycoumarin was used as internal standard (IS) and peaks were optimally separated using a Kinetex C18 column (2.6 μm, 150 mm Ã 2.10 mm I.D.) at 40 °C with an isocratic elution of mobile phase with sodium dihydrogen phosphate 0.01 M in water at pH 2.5 and methanol (55:45, v/v), at a flow rate of 0.25 mL/min. The injection volume was 10 μL. In all cases, the range of GEN recovery was higher than 61%. The low limit of quantification (LLOQ) was 25 ng/mL. The linearity of the calibration curves was satisfactory in all cases as shown by correlation coefficients >0.996. The within-day and between-day precisions were <15% and the accuracy ranged in all cases between 90.14% and 106.05%. This method was successfully applied to quantify GEN in liver, spleen, kidney and plasma after intravenous administration of a single dose (30 mg/kg) in female BALB/C mice
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volume 935, 15 September 2013, Pages 47-53
Journal: Journal of Chromatography B - Volume 935, 15 September 2013, Pages 47-53
نویسندگان
C. Tamames-Tabar, E. Imbuluzqueta, M.A. Campanero, P. Horcajada, M.J. Blanco-Prieto,