Shotgun analysis of membrane proteomes by an improved SDS-assisted sample preparation method coupled with liquid chromatography-tandem mass spectrometry

► Developed a simple SDS-assisted sample preparation method for shotgun analysis of membrane proteomes. ► Utilized the strong solubilization ability of SDS for the extraction and solubilization of membrane proteins. ► Optimized KDS precipitation for high efficiency of SDS-removal and peptide recovery. ► The developed method obviously improves the identification of membrane proteins particularly IMPs. ► The developed method is easy to operate at low cost.