Methodology for a rapid and simultaneous determination of total cysteine, homocysteine, cysteinylglycine and glutathione in plasma by isocratic RP-HPLC

► Rapid and simultaneous determination of four total aminothiols levels in plasma. ► RP-HPLC isocratic elution within 6 min of all analytes, including the IS. ► Validated method exhibits an excellent precision and recovery for all aminothiols. ► Method appropriated for high-throughput routine clinical analysis.