LC-MS/MS for the simultaneous analysis of arachidonic acid and 32 related metabolites in human plasma: Basal plasma concentrations and aspirin-induced changes of eicosanoids

► We developed simultaneous quantitation tool of 32 eicosanoids in human plasma. ► High recovery of all analytes was achieved by solid phase extraction. ► LC/MS/MS method afforded high sensitivity to detect pg level of eicosanoids. ► This method is suitable for eicosanoid profiling in human plasma.