Determination of tolperisone in human plasma by liquid chromatography/tandem mass spectrometry for clinical application

► A simple, rapid, and sensitive LC-MS/MS method for human plasma tolperisone was developed. ► The lower limit of quantification using 200 μL of human plasma was 0.5 ng/mL. ► This method allows for minimal sample processing. ► The developed and validated method has been successfully applied to a pharmacokinetic study in humans.