Application of nanoLC-MS/MS to the shotgun proteomic analysis of the nematocyst proteins from jellyfish Stomolophus meleagris

► We analyzed the nematocyst proteins of jellyfish Stomolophus meleagris by using the nanoLC-MS/MS with the shotgun proteomic method. ► A total of 181 proteins had been identified with the molecular weight ranging from 5268.06 to 843,487.57 and the pI from 4.49 to 11.39. ► The identified nematocyst proteins were analyzed by bioinformatic method including gene ontology (GO) annotation, pathways and gene network analysis.