کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7618986 | 1494140 | 2012 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Therapeutic drug monitoring of tacrolimus by liquid chromatography-tandem mass spectrometry: Is it truly a routine test?
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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![عکس صفحه اول مقاله: Therapeutic drug monitoring of tacrolimus by liquid chromatography-tandem mass spectrometry: Is it truly a routine test? Therapeutic drug monitoring of tacrolimus by liquid chromatography-tandem mass spectrometry: Is it truly a routine test?](/preview/png/7618986.png)
چکیده انگلیسی
Therapeutic drug monitoring of tacrolimus by high-performance liquid chromatography-tandem mass spectrometry has become standard practice. We report on the long-term (4.5 years) use of one such method. Whole blood samples (25 μL) were treated with zinc sulphate (100 μL) and acetonitrile containing ascomycin (internal standard, 250 μL). A high-performance liquid chromatography-tandem mass spectrometer operating in positive ion mode with an electrospray interface was used. Chromatography was performed on a TDM C18 cartridge column (10 mm Ã 2.1 mm, 10 μm, Waters) using a switch gradient. A total of 4029 batches were analyzed for tacrolimus; this comprised of 81950 analyses of which 61027 were patient samples. Calibration curves (1.0-50 μg/L) were run on 1765 occasions (mean r2 = 0.999; range r2 = 0.988-0.999). Inter-batch accuracy and imprecision of the method (2.5, 12.5 and 30.0 μg/L), when in routine use, was 97.6-98.5% and <8.0%, respectively (n = 4031). Evaluation of the method against other methods in an external quality control scheme revealed good agreement by linear regression analysis (y = 0.924x + 0.196, r2 = 0.985). The percentage difference between our results and that of all methods revealed a mean bias of â6.3% and a range of â33.3% to 11.1%. During the evaluation period, four batch failures occurred (0.1% failure rate) and greater than 1000 samples per analytical column was achieved. In conclusion, the described method is ideally suited as a routine test for tacrolimus in the clinical setting.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography B - Volumes 883â884, 1 February 2012, Pages 108-112
Journal: Journal of Chromatography B - Volumes 883â884, 1 February 2012, Pages 108-112
نویسندگان
Paul J. Taylor, Michael E. Franklin, Chun-Hui Tai, Peter I. Pillans,