Simultaneous optimization of the resolution and analysis time of flavonoids in reverse phase liquid chromatography using Derringer's desirability function

► Combination of chemometrics and HPLC was used for optimization of separation of flavonoids. ► Flavonoids from different classes were separated using an isocratic mode of HPLC. ► By using the isocratic mode we could overcome the limitations of gradient elution. ► Good separation of flavonoids was obtained in reasonable analysis time.