Quality characteristics and fertilizing ability of ram sperm subpopulations separated by partition in an aqueous two-phase system

► CCCD differentiates sperm subpopulations of different quality characteristics. ► These include apoptotic changes and capacitation state. ► Sperm recovered from a two-phase system are suitable for fertilization assays. ► Separated subpopulations differ in ability of binding to zona pellucida of oocytes.